Diamond’s physical and chemical properties such as chemical inertness, high thermal conductivity and optical transparency make it an attractive substrate for integrated sensing and signaling. Researchers have employed various functionalization strategies for diamond films. However the biological sensing applications specifically for microfluidic lab on a chip (LOC) devices have not been fully explored. Microfluidic polymer based systems are difficult to directly integrate active structure, e.g. sensors for temperature, impedance or flow and actuators like heaters and pumps. By examining the DNA binding to single nanocrystalline diamond films we will provide further insight as to possible future biosensing applications for microfluidics. In order to achieve binding the biomolecules must be covalently linked to the surface. This will be achieved by employing different approaches to characterize doped single nanocrystalline diamond surfaces using X-ray photoelectron spectra (XPS), hydrogen plasma and oxygen plasma.